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Sequenced 93 accessions encompassing 3 Coffea speceis (C. arabica, C. canephora, and C. excelsa) uncovering 15,367,960 single-nucleotide polymorphisms (SNPs). Huang 2020
Investigation of the evolutionary patterns of key galactomannan (GM) biosynthesis genes in Coffea canephora revealed a high rate of evolutionary variation among GM pathway genes. Ogutu 2020
Sequenced DNA bulks from 18 Coffea arabica genotypes with extreme high- or low-caffeine content to identify polymorphisms linked to caffeine content. Found 1444 nonsynonymous single nucleotide polymorphisms (SNPs) associated with caffeine content. Tran 2018
Analyzed the changes in the types of highly expressed transcripts during bean maturation. A greater number of transcripts were expressed at the yellow stage. Cheng 2018
Genetic analysis of cultivated and wild coffee accessions of Coffea arabica (using C. canephora as a reference) identified genomic regions associated with lipid, cafestol, and kahweol contents and cafestol/kahweol ratio. A great allelic richness in wild Ethiopian accessions was observed. Sant'Ana 2018
Long-read sequencing of tetraploid Coffea arabica produced a reference transcriptome including more of the diversity of full-length transcripts. Caffeine and sucrose genes were targeted for case analysis. Cheng 2017
Presentation of a phylogenetic hypotheis for the genus Coffea and evaluation of the evolution of caffeine synthesis. Hamon 2017
Transcriptome analysis of leaf, flower and perisperm fruit tissue development in Coffea arabica provided an important step for identifying candidate genes related to several coffee metabolic pathways, especially those related to fruit chemical composition and therefore beverage quality. Ivamoto 2017
Investigation of the molecular basis of coffee diterpenes cafestol and kahweol biosynthesis via reporting of the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome p450 genes. Ivamoto 2017
Description of TropGeneDB, a web database that manages genomic, genetic, and phenotypic information on tropical crops, including coffee. Ruiz 2017
Described the genomic resources available for the study of coffee quality and explored the potential for the application of next generation sequencing and association genetic analysis to advance coffee quality research. Tran 2016
The utilization of active transposable elements has considerable potential to unravel the evolutionary history and delimitation of closely related Coffea species. Roncal 2016
Analysis of the expression of the beta-tubulin and endo-beta-mannanase genes during different phases at the end of the development and in different tissues of Coffea arabica seeds (including during the cherry stage). Santos 2015
Characterization and annotation of 87 putative cytochrome P450s from Coffea arabica obstained from the Brazilian Coffee Genome Project and description of their transcriptional pattern in different tissues and coffee organs. Ivamoto 2015
Identification of molecular markers linked to drought-resistant root traits in Coffea canephora. Achar 2015
Development of the Coffee Genome Hub, an integrative genome information system that allows centralized access to genomics and genetics data and analysis tools to facilitate translational and applied research in coffee. The complete genome sequence of Coffea canephora is provided. Dereeper 2015
Genetic characterization of the differential transcriptional regulation of caffeine metabolism in Coffea arabica and C. canephora. Perrois 2015
Investigation of the expression of genes that codify the antioxidant enzymes catalase (CAT), dismutase superoxide (SOD), and polyphenol oxidase (PPO) during the pre- and post-physiological maturation phases in whole coffee seeds and in endosperms and embryos extracted from the seeds. Santos 2014
Comparative analysis of the transcriptomes of Coffea arabica and C. canephora with a focus on fruit development using publicly available expressed sequence tags (ESTs). Data is available from The Coffee Bean EST public database. Paschoal 2014
Report of novel and conserved microRNAs in Coffea canephora and C. arabica. 58 unique sequences belonging to 33 families were found, including 2 novel miRNAs never described before in plants. Loss-Morais 2014
Development of new simple sequence repeat (SSRs) based microsatellite markers for Coffea canephora using in-silico bioinformatics and streptavidin-biotin based enrichment approach. Hendre 2014
Characterization of the biosynthetic pathway of trigonelline. Production is likely catalyzed by N-methyltransferase. Mizuno 2014
Generation of a high-quality draft genome of Coffea canephora. Comparative analyses of caffeine N-methyltransferases demonstrate that these genes expanded through sequential tandem duplications independently of genes from cacao and tea, suggesting that caffeine in eudicots is of polyphyletic origin. Denoeud 2014
Botany. A wake-up call with coffee. [No abstract] Comment on: The coffee genome provides insight into the convergent evolution of caffeine biosynthesis. [Science. 2014] Zamir 2014
Development of a modified CTAB-based extraction method that allows for reliable isolation of high molecular weight genomic DNA from difficult to isolate plant species, including Coffea. Healey 2014
The changes in the linkage disequilibrium (LD) along the genome within the different Coffea canephora diversity groups was identified. In the different diversity groups studied, the LD was variable. Cubry 2013
The involvement of a novel intronic microRNA in cross regulation of N-methyltransferase genes involved in caffeine biosynthesis in Coffea canephora has been reported. Mohanan 2013
Metabolic phenotyping for the classification of coffee trees as per their metabolic fingerprints & the screening for families with desired characteristics, such as high or low caffeine content in their leaves by exploration of selection markers have been carried out. Montero-Vargas 2013
Functional characterization of three Coffea arabica L. monoterpene synthases: insights into the enzymatic machinery of coffee aroma. Del Terra 2013
Molecular AFLP and MSAP analyses and the phenotypic variation rate revealed high genetic and epigenetic stability in Coffea arabica plants derived from embryogenic suspensions and secondary embryogenesis. Bobadilla Landey 2013
Promoter analysis of the WRKY transcription factors CaWRKY1a and CaWRKY1b homoeologous genes in coffee (Coffea arabica) has been carried out. Petitot 2013
Comparison of structure of sorghum Hydroxycinnamoyl-transferase (HCT) with the HCT involved in chlorogenic acid synthesis in Coffea canephora revealed how CoA-dependent transferases with similar features can participate in different biochemical pathways across species. Walker 2013
The construction and characterization of a Bacterial Artificial Chromosome library from C. arabica Timor Hybrid CIFC 832/2, a parental genotype for several modern coffee cultivars has been reported. Cação 2013
Comparative genome mapping revealed that BAC-end sequences analysis provides first insights into coffee (Coffea canephora P.) genome composition and evolution. Dereeper 2013
It has been shown that subgenomes contribute to the transcriptome and their intertwined regulation in the allopolyploid Coffea arabica grown at contrasted temperatures. Combes 2013
Genetic variation and risks of introgression in the wild Coffea arabica gene pool in south-western Ethiopian montane rainforests have been evaluated. Aerts 2013
The genetic diversity and genetic structure of Coffea across Africa and the Indian Ocean islands was investigated. Across Africa and the Indian Ocean islands Coffea comprises a closely related group of species with an overall pattern of genotypes running from west to east. Razafinarivo 2013
A study on the physicochemical characteristics and antioxidant activity of roasted and non-roasted coffee extracts revealed activation of antioxidant response element dependent genes by roasted coffee extracts. Yazheng 2012
CaPrx, a Coffea arabica gene encoding a putative class III peroxidase showed enhanced expression in coffee roots inoculated with root-knot nematode infection. (at 12 h post-inoculation), but no significant difference in expression was observed between susceptible and resistant plants. Severino 2012
The cloning and overexpression of hydroxyl-cinnamoyl transferase from Coffea canephora in Escherichia coli as well as its purification and crystallization are presented. Lallemand 2012
It is shown that significant conservation is found between distantly related species from the Asterid (Coffea canephora and Solanum sp.) and Rosid (Vitis vinifera) clades, at the genome macrostructure and microstructure levels. Guyot 2012
A molecular- and bioinformatic-based search for Coffea canephora Phenylalanine ammonia lyase 1 (CcPAL1) paralogues resulted successfully in identifying two additional genes, CcPAL2 and CcPAL3, presenting similar genomic structures and encoding proteins with close sequences. Lepelley 2012
Study examined the molecular mechanisms underlying drought acclimation in coffee plants by the identification of candidate genes. Factors involved a complex network of responses perhaps involving the abscisic signalling pathway and nitric oxide may be major molecular determinants. Marraccini 2012
Apoatã and Coffea dewevrei seemed to share closer parental origins to Coffea canephora cv. Robusta, whereas Icatu, Obatã and Catuaí showed higher similarities to Coffea arabica. Bicho 2012
A gene (HhMAN1), identified from the coffee berry borer beetle, Hypothenemus hampei, a devastating pest of coffee, shows clear evidence of horizontal gene transfer from bacteria. Acuña 2012
A study on genetic diversity of Coffea arabica L. in Nicaragua as estimated by simple sequence repeat markers showed that the genetic variation in Nicaraguan coffee is significant enough to be used in the breeding programs. Geleta 2012
Two hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyl transferases (HCT/HQT) from coffee were biochemically characterized. Lallemand 2012
The paper provides a detailed account of advances made in genetic transformation of coffee and their potential applications. Mishra 2012
The pCa06 clone, containing a fragment of 775 bp length, was characterized by sequencing and used as a probe in chromosomes of Coffea arabica and six other species: C. canephora, Coffea eugenioides, Coffea kapakata, Coffea liberica var. dewevrei, Coffea racemosa, and Coffea stenophylla. Yuyama 2012
Synonymous codon usage of 53 protein coding genes in chloroplast genome of Coffea arabica was analyzed for the first time to find out the possible factors contributing codon bias. Nair 2012
The study investigated the influence of growing temperatures on homeologous gene expression in Coffea arabica L., a recent allopolyploid involving 2 closely related diploid parental species. Combes 2012
Three Coffea orthologous regions of 470-900 kb were analyzed & compared: both subgenomes of allotetraploid Coffea arabica (contributed by the diploid species C. eugenioides and C. canephora) & the genome of diploid C. canephora. Cenci 2012
Described the cloning and the biochemical and molecular characterization of Coffea arabica ß-galactosidase expressed in the pericarp and the endosperm of coffee fruits in all phases of ripeness. Figueiredo 2011
Characterized and described the expression of two cDNA encoding 3-Hydroxy-3-methylglutaryl coenzyme A reductase isoforms in coffee (Coffea arabica L.). CaHMRG2 was expressed in all tissues and during all fruit development stages examined. Tiski 2011
Two novel Ty1-copia retrotransposons isolated from coffee trees can effectively reveal evolutionary relationships in the Coffea genus (Rubiaceae). Hamon 2011
"PUCE CAFE' Project: the first 15K coffee microarray will help study functional genomics in a wide range of experiments on various plant tissues, such as analyzing coffee bean maturation or resistance to pathogens or drought. Privat 2011
Flow cytometric analysis using SYBR Green I for genome size estimation in coffee. Study found genome size measured from nuclei stained with SYBR Green I and propidium iodide was statistically identical. RonildoClarindo 2011
Study found Agrobacterium-mediated transformation of embryogenic cultures a viable and useful tool both for coffee breeding and for the functional analysis of agronomically important genes. Ribas 2011
To understand the physiological role & regulation of the expansin gene (EXP) family in Coffea arabica fruits during growth & maturation, 11 expansin genes, were identified nine belonging to the α-expansin family (EXPA), one EXLA and one EXLB, through in silico analysis of expressed sequence tags. Budzinski 2011
The inhibition of DNA methyltransferase 3a by dietary polyphenols from black tea and coffee was studied. Data suggests a biochemical mechanism for health effects of black tea and coffee and a possible molecular mechanism for the improvement of brain and mental health by dietary polyphenols. Rajavelu 2011
In a study of disease resistance in coffee trees, sequence analysis of the SH3 region in three coffee genomes, Ea and Ca subgenomes from the allotetraploid C. arabica and Cc genome from the diploid C. canephora, revealed the presence of 5, 3 and 4 R genes in Ea, Ca, and Cc genomes, respectively. Ribas 2011
The study revealed the existence of two homeologous RBCS1 genes in Coffea arabica: one carried by the C. canephora sub-genome (called CaCc) and the other carried by the C. eugenioides sub-genome (called CaCe). Marraccini 2011
The mechanism of Coffea gametophytic self-incompatibility (GSI) was investigated and the potential for homology to S-RNase GSI by sequencing class III RNase T2 genes in populations of 14 African & Madagascan Coffea species was examined. Nowak 2011
Study was carried out for the characterization and expression of two cDNA encoding 3-Hydroxy-3-methylglutaryl coenzyme A reductase isoforms in coffee (Coffea arabica L.). Tiski 2011
Study was carried out to describe genomic expression dominance between a relatively recently formed natural allopolyploid (Coffea arabica) and its ancestral parents (Coffea canephora and C. eugenioides) and to determine if the divergence was environment-dependent. Bardil 2011
The Miniature Inverted-repeat Transposable Elements family will enable the study of the Coffea canephora genome evolution, phylogenetic relationships, and possible gene flows within the Coffea genus. Dubreuil-Tranchant 2011
The study comprised 24 coffee accessions based on their genetic origin including: arabica accessions (6 genotypes of C. arabica), resistant arabica (6 leaf rust-resistant C. arabica genotypes with introgression of Híbrido de Timor), robusta (5 C. canephora genotypes). Missio 2011
Identification and characterization of the Non-race specific Disease Resistance 1 (NDR1) orthologous protein in coffee. Cacas 2011
The work aimed to study the control of the biosynthesis of antinutritional factor phytate & its associated Fe-rich protein family, ferritin, in coffee & the Transcriptional profile of genes involved in the biosynthesis of phytate & ferritin in Coffea were studied. Nobile 2010
It is shown that the obtainment of viable coffee plantlets, transformed by bombardment with the cry1ac gene and without selection markers nor reporter genes, is feasible. De Guglielmo-Cróquer 2010
Comparative sequence analyses indicate that Coffea (Asterids) and Vitis (Rosids) derive from the same paleo-hexaploid ancestral genome. Cenci 2010
The possible genetic effect on vegetative propagation of Coffea canephora was evaluated. The observations led to conclude that somatic embryogenesis and cutting abilities of C. canephora Pierre appeared to be genetic dependent but through independent mechanisms. Priyono 2010
Developing Coffea arabica seeds accumulate large amounts of chlorogenic acids (CGAs) as a storage form of phenylpropanoid derivatives, making coffee a valuable model to investigate the metabolism of these widespread plant phenolics. Joët 2010
A high-throughput data mining of single nucleotide polymorphisms in Coffea species expressed sequence tags suggests differential homeologous gene expression in the allotetraploid Coffea arabica. Vidal 2010
The investigation assessed the resistance of 12 coffee genotypes to the leaf miner Leucoptera (= Perileucoptera) coffeella & correlated such results with the leaf content of coffee alkaloids & phenolics that probably play a role in the interaction between coffee and the leaf miner. Magalhães 2010
A set of 127 microsatellite markers known as SSRs (Simple Sequence Repeats), was used to analyze genetic similarity in 25 Coffea arabica varieties. These were composed of 19 commercially important Brazilians & 6 interspecific hybrids of Coffea arabica, C. canephora & C. liberica. Vieira 2010
Studied the biosynthesis of caffeine by examining the expression of genes involved in its biosynthetic pathway in coffee fruits containing normal or low levels of caffeine. Maluf 2009
Coffea canephora of Ugandan origin was found to be genetically different from previously identified diversity groups, implying that it forms another diversity group within the species. Musoli 2009
Both microsatellite (simple sequence repeat, SSR) and restriction fragment length polymorphism (RFLP) markers were used to investigate the genetic variation pattern of Coffea canephora in the Guineo-Congolean distribution zone. Gomez 2009
The study provides a first glimpse of Coffea canephora genome composition and evolution and the data revealed a remarkable conservation of the micro-collinearity between C. canephora and Vitis vinifera and a high conservation with other distant dicotyledonous reference genomes. Guyot 2009
The study was conducted in order to calculate the Coffea canephora and Coffea arabica chromosomal DNA content, associating cytogenetic methodologies with flow cytometry and image cytometry tools. Clarindo 2009
The biosynthesis of caffeine was studied by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. Maluf 2009
Expression studies demonstrated that a cDNA clone (designated CaIRL) is expressed exclusively in coffee leaves (Coffea arabica) and its transcript level is markedly increased in response to fungal infection and mechanical injury. Brandalise 2009
The chromosome organization among 15 wild diploid Coffea species and cultivated tetraploid C. arabica was determined by fluorochrome banding (CMA, DAPI) and double fluorescence in-situ hybridization (FISH) of 5S and 18S rDNA achieved on the same chromosome plates. Hamon 2009
The study was carried out to reconstruct the metabolic pathways involved in the biosynthesis of the main coffee seed storage compounds, namely cell wall polysaccharides, triacylglycerols, sucrose, and chlorogenic acids. Joët 2009
The aim of the research was to compare the relative expression of a set of potential reference genes across different types of tissue/organ samples of coffee & the data suggests that the expression of housekeeping genes is not completely stable in coffee. Barsalobres-Cavallari 2009
Coffea arabica L. plants grow in acidic soils & thus aluminium (Al) toxicity is a major negative impact on crop productivity. To understand Al toxicity mechanisms via Al absorption kinetic, protoplasts from two C. arabica cell lines: Al-sensitive & Al-tolerant were isolated. Ramírez-Benítez 2009
Work was carried out to isolate & characterize cDNA encoding proteins responsible for galactomannan synthesis in coffee & to study the expression of corresponding transcripts in developing coffee grain from C. arabica & C.canephora, which exhibits galactomannan variations. Pré 2008
The study was aimed to develop new coffee-specific SSR markers and validate their utility in analysis of genetic diversity, individualization, linkage mapping, and transferability for use in other related taxa. Hendre 2008
The study shows transferability of microsatellite markers developed in the two cultivated coffee species (Coffea arabica L. and C. canephora Pierre ex Froehn.) to 15 species representing the previously identified main groups of the genus Coffea. Cubry 2008
The incorporation of chlorogenic acids and their subunits quinic and caffeic acids in coffee brew melanoidins was studied. Bekedam 2008
Transposable elements in Coffea (Gentianales: Rubiacea) transcripts and their role in the origin of protein diversity in flowering plants. Lopes 2008
The study was carried out to decipher the transcriptional networks that govern the development of the Coffea arabica seed, a model for non-orthodox albuminous seeds of tropical origin. Salmona 2008
The potential of using nitrogen-nonutilizing mutants and vegetative compatibility groups to characterize the genetic variability of the Cercospora coffeicola population associated with coffee plantings in Brazil was evaluated. Martins 2008
Through gene expression and enzyme activity analysis Coffea canephora,(Robusta) was found to accumulate less sucrose than Coffea arabica. Privat 2008
A cDNA encoding the catalytic site of a phosphatidyl-inositol-specific phospholipase C (PI-PLC) was isolated from Coffea arabica suspension cells. The cDNA encodes a polypeptide of 308 amino acids, containing the catalytic X and Y domains, and has 99% identity to the soybean gene. Sánchez-Cach 2008
The chemotaxonomic relationships between African Coffea species were assessed on the basis of their seed lipid composition. Fatty acids and sterols were determined in 59 genotypes belonging to 17 distinct Coffea species/origins to establish the phylogenetic relationships. Dussert 2008
Molecular characterization of three full-length cDNAs corresponding to a putative ethylene receptor gene (CcETR1) isolated from Coffea canephora cDNA libraries, and the relationship of CcETR1 with fruit development and caffeine content. Bustamante-Porras 2007
A set of 40 polymorphic microsatellite markers was developed using a GA/GT-enriched Coffea canephora genomic library. Amplification of these markers was tested in accessions of C. heterocalyx & C. pseudo-zanguebariae belonging to different African geographical clades. Poncet 2007
The genes encoding XMT and DXMT, the enzymes from Coffea canephora (robusta) that catalyse the three independent N-methyl transfer reactions in the caffeine-biosynthesis pathway, have been cloned and the proteins have been expressed in Escherichia coli. McCarthy 2007
It is suggested that phosphatidic acid (PA) formation was prevented through the inhibition of the Phospholipase C activity, and it provides the first evidence for the role of Aluminum (Al(3+)) toxicity on PA production. Ramos-Díaz 2007
The accumulation of chlorogenic acids and free quinic acids was studied in Coffea arabica cv. Tall Mokka & Coffea canephora seeds. Study results showed that biosynthetic activity of chlorogenic acids was clearly reduced in ripening and ripe seeds, especially in C canephora. Koshiro 2007
Comparative sequencing of > 7 kb of highly variable chloroplast genome regions (atpB-rbcL, trnS-trnG, rpl22-rps19 & rps19-rpl2 spacers; introns in atpF, trnG, trnK, and rpl16) with microsatellites known from other angiosperms was carried out in Coffea. Tesfaye 2007
The relationships between Coffea and Psilanthus species are assessed based on substantially increased molecular sequence data and greatly improved species sampling. Maurin 2007
Fluorescence in situ hybridization analysis is a tool for molecular cytogenetic studies in coffee, providing rapid localization of either specific chromosomes or alien chromatin in introgressed genotypes derived from diploid species displaying substantial genomic differentiation from C. arabica. Herrera 2007
The processes governing hybrid formation and introgression between the allotetraploid Coffea arabica and one of its ancestral diploid progenitors, C. canephora, in a sympatric zone of New Caledonia was investigated. Mahé 2007
The availability of the complete chloroplast genome of coffee provides regulatory and intergenic spacer sequences for utilization in chloroplast genetic engineering to improve this important crop. Samson 2007
Identification, characterization and utilization of EST-derived genic microsatellite markers for genome analyses of coffee and related species. Aggarwal 2007
Hydroxy cinnamoyl quinic acid (HQA) biosynthesis & accumulation occurred mainly in shoot tip in phloem of vascular bundles. The lack of correlation between gene expression & HQA content observed in some organs is discussed in terms of transport & accumulation mechanisms. Mahesh 2007
The aluminum (Al)-sensitive Coffea arabica suspension cell line L2 have been used to analyse effect of 2 different Al species on the phosphoinositide signal transduction pathway. The results have shown that the association of Al with coffee cells was affected by the pH & the form of Al in media. Quintal-Tun 2007
Isolated a full-length cDNA (pF6), corresponding to a phenylalanine ammonia-lyase (PAL) gene (CcPAL1) by screening a Coffea canephora fruit cDNA library. Characterized its corresponding genomic sequence. Mahesh 2006
Biochemical and genomic analysis of sucrose metabolism and the role of sucrose synthase in the fruit tissues (pericarp, perisperm, and endosperm) of Coffea arabica during development. Sucrose synthase showed the highest activities during the last stage of endosperm and pericarp development. Geromel 2006
It is found that Coffea expressed sequence tags (ESTs) are a valuable resource for microsatellite mining. EST-simple sequence repeat markers developed from Coffea canephora sequences can be easily transferred to other Coffea species for which very little molecular information is available. Poncet 2006
In anti-sense and RNA interference transgenic plants of Coffea canephora in which the expression of CaMXMT1 was suppressed, caffeine biosynthesis from [8-(14)C]adenine was investigated, together with the overall metabolism of [8-(14)C]adenine. Ashihara 2006
A full-length cDNA (pF6), corresponding to a phenylalanine ammonia-lyase (PAL) gene (CcPAL1), was isolated by screening a Coffea canephora fruit cDNA library and its corresponding genomic sequence was characterized. Mahesh 2006
A comparative evaluation of a few methods of ligation-mediated polymerase chain reaction methods and a ligation-independent one were made by isolating promoter fragment for N-methyltransferase gene involved in the caffeine biosynthetic pathway of Coffea canephora. Satyanarayana 2006
The isolation, characterization and quantitative expression analysis of six cDNAs representing five genes of the oleosin family of Coffea canephora (CcOLE-1 to CcOLE-5) and one gene of the steroleosin family (CcSTO-1) were presented. Simkin 2006
The genetic transformation of Coffea canephora by co-cultivation with Agrobacterium rhizogenes harbouring a binary vector was developed & found that A. rhizogenes can be used for delivering transgenes into tree species Coffea using binary vectors with A. tumefaciens T-DNA borders. Kumar 2006
Screening of effective food-processing cellulase for digestion of cell walls of coffee beans was carried out. Kasai 2006
Though the differences in chlorogenic acids & 1,5-gamma-quinolactones contents of regular & decaffeinated roasted arabica coffees appear to be relatively small, they may be enough to affect flavor characteristics as well as the biopharmacological properties of the final beverage. Farah 2006
Two Agrobacterium mediated transformation techniques; the first with A. tumefaciens to introduce an insect resistance gene & the second with A. rhizogenes to study candidate gene expression for nematode resistance in transformed roots in coffee plants were described. Leroy 2006
Several full-length cDNA-encoding dehydrins (CcDH1, CcDH2 and CcDH3) and an LEA protein (CcLEA1) from Coffea canephora (robusta) were isolated and characterized with the aim of characterizing and studying genes involved in osmotic stress tolerance in coffee. Hinniger 2006
The role of phenolic compounds, and the enzymes peroxidase and polyphenol oxidase, in the expression of resistance of coffee plants to Leucoptera coffeella were examined and found not to show strong evidence of their participation in direct defensive mechanisms. Ramiro 2006
Based on expression studies of the germination-specific isocitrate lyase & resumption of cell cycle activity, monitored by the abundance of beta-tubulin, it is evidenced that germination is initiated in coffee seeds during course of standard coffee post-harvest treatments. Selmar 2006
The effects of benzo(1,2,3)thiadiazole-7-carbothioic acid-S-methyl ester (BTH), a SAR chemical inducer, on the expression profile of Coffea arabica was reported. De Nardi 2006
The first bacterial artificial chromosome library of Robusta coffee (Coffea canephora) was constructed, with the aim of developing molecular resources to study the genome structure and evolution of this perennial crop. Leroy 2005
This is the first report of cloning of the promoter for a N-Methyltransferases gene involved in caffeine biosynthetic pathway in Coffea canephora and it opens up the possibility of studying the molecular mechanisms that regulate the production of caffeine. Satyanarayana 2005
It is demonstrated that PCR-grade DNA may be obtained from roasted beans and even instant coffee, which would allow analysis of commercial samples, provided that suitable markers for species/variety identification are found. Martellossi 2005
The study was carried out to identify which nonvolatile(s) of coffee are responsible for the interactions observed between nonvolatile coffee brew constituents and thiols, sulfides, pyrroles, and diketones. Charles-Bernard 2005
The influence of coffee roasting on the thiol-binding activity of coffee beverages and the potential of various green bean compounds as precursors of thiol-binding sites by using promising "in bean" model roast experiments were investigated. Müller 2005
It is suggested that polyamines, may modulate the cellular signal of Coffea arabica cells by differentially affecting components of the phospholipid cascade. Echevarría-Machado 2005
Coffee & tomato have a similar genome size, chromosome karyotype (tomato, n=12; coffee n=11) & chromosome architecture. Both belong to Asterid I clade of dicot families. Their biology may be united into one common network of shared discoveries, resources & information. Lin 2005
A different 1089 bp cDNA open reading frame encoding alpha-D-galactosidase (alpha-Gal) of Coffea liberica & C. canephora was cloned by homology-based RT-PCR. The cloned Gal most closely resembles the corresponding one from C. arabica. [Article in Chinese] Liang 2005
Genetic variation of forest coffee trees (Coffea arabica L.) from four regions of Ethiopia was investigated using inter-simple sequence repeat (ISSR) markers. A total of 160 individuals representing 16 populations were sampled. Aga 2005
Two species of the genus Coffea, C. arabica (Colombia) & C. canephora (Indiano Robusta) were analysed by two-dimensional (2-D) maps in order to obtain fingerprints of the expressed polypeptide chains and to determine which ones would characterize the two species. Gil-Agusti 2005
Conversion of the amplified fragment length polymorphism markers into sequence-characterized amplified region (SCAR) anchor markers allowed assessing sequence conservation within Coffea species with respect to species relatedness. Poncet 2005
Report of an expressed sequence tags analysis provides a useful resource for studying coffee resistance responses and for improving C. arabica for durable disease resistance. Fernandez 2004
About 80% of Aspergilluscarbonarius strains collected from coffee beans is able to produce OchratoxinA (OTA) on this substrate. The PCR assay could contribute to the early and rapid detection of the potential presence of OTA in coffee samples. Fungaro 2004
The work was carried out to gain insight into the mechanism of introgression into Coffea arabica of a leaf rust resistance gene from C. liberica (i.e. S(H)3 resistance factor) and to identify associated molecular markers. Prakash 2004
55 percent of the alleles found in the wild tetraploids were not shared with cultivated C. arabica genotypes, supporting the idea that wild tetraploid ancestors from Ethiopia could be used as a source of genetic variation to expand the gene pool of elite C. arabica germplasm. Moncada 2004
Samples of Coffea arabica were collected during the different stages of the fermentation from two production sites in Tanzania. The yeasts community was identified by genotyping using ITS-PCR and sequence analysis of the D1/D2 domain of the 26S rRNA gene. Masoud 2004
Aspergillus ochraceus can be rapidly and specifically detected in green coffee by polymerase chain reaction. A positive correlation between the ochratoxin A content and the DNA quantity was established. Schmidt 2004
The ability of tubulin-based polymorphism (TBP) to discriminate between accessions & species in oilseed rape, coffee & lotus is shown. The TBP was able to detect genetic polymorphisms in the context of a simplified & readily appreciable pattern of DNA amplification. Bardini 2004
In order to promote genome research on coffee trees, one of the most important tropical crops, a bacterial artificial chromosome (BAC) library of the coffee allotetraploid species, Coffea arabica, was constructed. Noir 2004
AFLPs have been used to construct a genetic linkage map on a pseudo-F(2) population of arabica coffee (Coffea arabica L.) derived from a cross between the cultivars Mokka hybrid and Catimor. Pearl 2004
The major noncellulosic polysaccharides and proteoglycans in the coffee bean (Coffea arabica) cell wall are (galacto) mannans & arabinogalactan proteins (AGP). Immunological & chemical probes demonstrated that the mannans AGP proteins were located across the width of the cell wall. Sutherland 2004
Factors controlling gene introgression into cultivated Coffea arabica L. were investigated. Interspecific triploid hybrid plants between the tetraploid species C. arabica & a diploid species, either C. canephora or C. eugenioides, were backcrossed to C. arabica (male parent). Herrera 2004
Application of RNAi to confirm theobromine as the major intermediate for caffeine biosynthesis in coffee plants with potential for construction of decaffeinated varieties. Ogita 2004
Isolated three types of cDNAs encoding N-methyltransferases from immature fruits of coffee (Coffea arabica) plants, and designated as CaXMT1, CaMXMT2, and CaDXMT1, respectively. Uefuji 2003
The genetic parameters of several agronomic traits were estimated in Coffea canephora in a triangular diallel with six parents and in a partial diallel with 18 parents. Cilas 2003
Self-compatibility segregation was assessed in two successive backcross progenies originating from an interspecific cross between Coffea canephora (self-incompatible) and Coffea heterocalyx (self-compatible). Coulibaly 2003
DNA from Coffea arabica leaves was used for RAPD analysis and a total of 144 leaf samples collected from 16 provenances in five regions of Tanzania were analysed. Masumbuko 2003
Effects of caffeine and chlorogenic acid on propidium iodide accessibility to DNA: consequences on genome size evaluation in coffee tree. Noirot 2003
It is suggested that chlorogenic acids (CGA) s.s. accumulation is dependent on the caffeoyl-coenzyme A 3-O-methyltransferase allele present and consequently on the activity of the encoded isoform, whereby CGA accumulation increases as the isoform activity decreases. Campa 2003
The full-length cDNA of coffee tentative caffeine synthase 1, CtCS1, previously isolated by the rapid amplification of cDNA ends was translated with an Escherichia coli expression system and the resultant recombinant protein was purified using Ni-NTA column. Mizuno 2003
A total of 75 polymorphic RAPD bands generated by twelve random primers were used to assess genetic diversity among 144 genotypes representing 16 C. arabica populations. Aga 2003
Coffee samples from pure lines, compared in a Trial 1, and from F1 hybrids and parental lines from a half-diallel trial in a Trial 2, were studied for beverage quality, chemical composition and amount of introgressed genetic material. Bertrand 2003
The amino acid sequence of a 26 kDa basic subunit of 13S globulin of common buckwheat (Fagopyrum esculentum Moench) was described. The protein has 93 and 75% sequence homology with 11S globulin of Coffea arabica and beta subunit of 11S globulin of Cucurbita pepo respectively. Bharali 2003
The study on how age of embryogenic cell suspensions affects somaclonal variation in five F1 hybrids of Coffea arabica L was determined. Seven phenotypic variants were characterized. Etienne 2003
Amplified fragment-length polymorphism was used to evaluate the stability of DNA in regenerated plantlets of Coffea arabica obtained by direct and indirect somatic embryogenesis. Sanchez-Teyer 2003
Isolation of a new dual-functional caffeine synthase gene encoding an enzyme for the conversion of 7-methylxanthine to caffeine from coffee (Coffea arabica L.). Mizuno 2003
The effect of aluminium (Al) on phosphoinositide-specific phospholipase C (PLC)& lipid kinase activities was examined in a cellular suspension of coffee. Al disrupts metabolism of membrane phospholipids regulating PLC & other enzymes involved in signal-transduction pathways. Martínez-Estévez 2003b
Characteristics changes between the wild and Al-tolerant coffee cell lines are examined. Martínez-Estévez 2003a
In a study examining changes to the galactose/mannose ratio in galactomannans during Arabica bean development found the degree of substitution of galactomannans in coffee beans is developmentally regulated, possibly due to the action of an alpha-galactosidase. Redgwell 2003
In order to establish a successful method of genetic transformation in Coffea arabica cv. Catimor, different conditions of generation and electroporation were evaluated on different plant tissues. [Article in Spanish] Fernández Da Silva 2003
The genome size of coffee trees (Coffea sp.) was assessed using flow cytometry and an accurate estimate of genome size was obtained with propidium iodide. Noirot 2002
A differential display analysis approach was employed to investigate the changes taking place during the induction of somatic embryogenesis in leaf explants and suspension cultures of coffee. Roja-Herrera 2002
Pollen viability restoration in a Coffea canephora P. and C. heterocalyx Stoffelen backcross. QTL identification for marker-assisted selection. Coulibaly 2002
Transfer of desired characters from the diploid relative species such as Coffea canephora into the cultivated allotetraploid coffee species ( Coffea arabica L.) is essential to the continued improvement of varieties such as intergenomic recombination events and gene introgressions. Herrera 2002
Eleven Coffea arabica accessions derived from the disseminated bases were evaluated by amplified fragment length polymorphism using 37 primer combinations and simple-sequence repeats produced by 6 microsatellites. Anthony 2002
Genetic diversity of Coffea arabica cultivars was estimated using AFLP markers. 61 Coffea accessions comprising 6 arabica cultivars, including Typica, Bourbon, Catimor, Catuai, Caturra & Mokka Hybrid & 2 diploid Coffea sp. were analyzed with 6 EcoRI- MseI primer combinations. Steiger 2002
Gene introgression into Coffea arabica by triploid hybrids (C. arabica x C. canephora) reveal a strong deficiency in C. canephroa alleles indicating a severe counter-selection against introgression of genetic material from C. canephora into C. arabica by way of triploid hybrids. Herrera 2002
The cloning and sequencing of two endo-beta-mannanase cDNAs (manA and manB) by different strategies from Coffea arabica L. was reported. Marraccini 2001
5-Fluoro-alpha-D-galactopyranosyl fluoride was synthesized and its interaction with the active site of an alpha-galactosidase from green coffee bean (Coffea arabica), a retaining glycosidase, characterized kinetically and structurally. Ly 2000
Polyphenol oxidase (PPO) was characterized in partially purified extracts of leaves and fruit endosperm of coffee (Coffea arabica L.). PPO activity was higher in early developmental stages of both leaves and endosperm of fruits. Mazzafera 2000
[Characterization of microsatellite loci in Coffea arabica and related coffee species]. No abstract available. Combes 2000
Single-locus inheritance in the allotetraploid Coffea arabica L. and interspecific hybrid C. arabica x C. canephora. Lashermes 2000
The degradation of xanthine was studied in young and aged leaves and in immature and mature fruits of Coffea arabica and Coffea dewevrei, which differ with respect to caffeine catabolism. Vitória 1999
Restriction fragment length polymorphism (RFLP) markers were used in combination with genomic in situ hybridisation (GISH) to investigate the origin of the allotetraploid species Coffea arabica (2n = 44). Lashermes 1999
The trnL-trnF intergenic spacer of cpDNA has been sequenced from 38 tree samples representing 23 Coffea taxa and the related genus Psilanthus. These sequences were used for phylogenetic reconstruction using parsimony analyses. Cros 1998
The anion conductance of the plasma membrane of Coffea arabica protoplasts was isolated and characterized using the whole-cell patch clamp technique. Voltage pulse protocols revealed two components: a voltage-gated conductance (Gs) and a voltage-independent one (Gl). Dieudonné 1997
[A cDNA encoding a metallothionein I-like protein from coffee leaves (Coffea arabica)]. No abstract available. Moisyadi 1995
A technique was developed to purify and characterize an isozyme alpha-D-galactosidase from Coffea canephora to homogeneity. The isolated enzyme has a molecular weight of 36.7 kDa by SDS PAGE and 34.0 kDa by gel filtration. Haibach 1992
History of Record
ORIGINAL RESEARCH BY: J. Mohanasundaram, MD, PhD
April 2013
May 2019
LATEST UPDATES BY: Oren Rabinowitz, MSc
December 2020